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KMID : 0903619940350030213
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Journal of the Korean Society for Horticultural Science
1994 Volume.35 No. 3 p.213 ~ p.219
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Studies on Rapid In Vitro Multiplication of Potato (Solanum tuberosum L.) Microtubers and Their Practical Use
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Abstract
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The relationship between tuber-inducing media and dormancy period of microtubers formed in vitro was studied. Plantlet culture conditions and sucrose concentrations in tuber-inducing medium did not affect the dormant period of microtubers. The length of dormant period was determined largely by the size of microtubers: larger tubers sprouted earlier. Percent sprouting was over 80% when microtubers were larger than 0.5 cm in diameter. Fifty percent of ´Dejima´ microtubers sprouted after 30 days of harvest. The dormancy period of microtubers from TI-5 combined with 100 times strength of vitamines of MS basic medium and 9% sucrose was shorter than those from TI-3 combined with 100 times strength of vitamines of MS basic medium, 6% sucrose, 500 ㎎/ℓ CCC and 5 ㎎/ℓ BAP. Dormancy period of microtubers was altered by tuberinducing media. Early sprouting and the highest sprouting rate of microtubers were observed with the microtubers of ´Dejima´ from TI-5 medium, while in ´Irish Cobbler´, early sprouting was obtained with microtubers from TI-4 medium but the kinds of media did not affect the final percent of sprouting. KMnO₄ added to tuber-inducing medium also shortened dormancy peroid of microtubers at 10 and 20 μM with ´Dejima´ and 20 μM with ´Irish Cobbler´. Soaking of in vitro microtubers in 1000 ppm GA₃ solutions for one hour broke dormancy of microtubers.
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